rrid p p42 44 thr202 tyr204 rabbit cell signaling technology 4377 ab 331775 p jnk  (Cell Signaling Technology Inc)

Journal: HemaSphere

Article Title: Hepatocyte Toll‐like receptors contribute to the hepcidin inflammatory response to pathogens and pathogen‐derived ligands

doi: 10.1002/hem3.70096

Figure Lengend Snippet: List of antibodies used for western blot.

Article Snippet: 44/42 MAPK (ERK1/2) , 1:1000 , 4695, Cell Signaling.

Techniques: Western Blot

Journal: HemaSphere

Article Title: Hepatocyte Toll‐like receptors contribute to the hepcidin inflammatory response to pathogens and pathogen‐derived ligands

doi: 10.1002/hem3.70096

Figure Lengend Snippet: The MAPK pathways JNK and ERK are involved in hepatocyte Hamp upregulation in response to PAMPs and pathogens. (A) Primary murine hepatocytes were treated with FSL1, TNF, or Insulin for the indicated time points. Western blot analyses of phospho‐JNK, phospho‐p38, phospho‐ERK, and phosho‐AKT. Vinculin, total JNK, and total p38 were used as loading controls. Primary murine hepatocytes were pre‐treated for 1 h with (B) BI605906, (C) SB203580, (D) SP600125, (E) Wortmannin, or (F) U0126 and then stimulated with FSL1 for 4 h. The mRNA expression of Hamp was analysed by qRT‐PCR analysis. Primary murine hepatocytes were pre‐treated for 1 h with (G, I) SP600125 or (H, J) U0126 and then stimulated with (G, H) heat‐killed S. aureus , B. abortus , or (I, J) FLA‐ST for 4 h. The mRNA expression of Hamp was analysed by qRT‐PCR analysis. mRNA expression data were normalized to the housekeeping gene Rpl19 . One representative experiment with four technical replicates. Data are reported as mean ± SD. Two‐way ANOVA, followed by Sidak's and Dunnett's multiple comparison tests. */ # Refers to comparisons with the respective non‐treated controls and with the matched treatment in vehicle controls, respectively: * /# p < 0.05, ** /## p < 0.01, *** /### p < 0.001, **** /#### p < 0.0001. INS, Insulin; NT, non‐treated; p, phospho; RQ, relative quantification; t, total.

Article Snippet: 44/42 MAPK (ERK1/2) , 1:1000 , 4695, Cell Signaling.

Techniques: Western Blot, Expressing, Quantitative RT-PCR, Comparison, Quantitative Proteomics

Journal: HemaSphere

Article Title: Hepatocyte Toll‐like receptors contribute to the hepcidin inflammatory response to pathogens and pathogen‐derived ligands

doi: 10.1002/hem3.70096

Figure Lengend Snippet: List of antibodies used for western blot.

Article Snippet: Phospho‐44/42 MAPK (ERK1/2) , 1:1000 , 4370, Cell Signaling.

Techniques: Western Blot